Z probe, an efficient tool for characterizing long non-coding RNA in FFPE tissues

Manish Tripathi, Chidi Zacheaus, Kyle Doxtater, Fatemeh Keramatnia, Cuilan Gao, Murali Yallapu, Meena Jaggi, Subhash Chauhan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Formalin-fixed paraffin embedded (FFPE) tissues are a valuable resource for biomarker discovery in order to understand the etiology of different cancers and many other diseases. Proteins are the biomarkers of interest with respect to FFPE tissues as RNA degradation is the major challenge in these tissue samples. Recently, non-protein coding transcripts, long non-coding RNAs (lncRNAs), have gained significant attention due to their important biological actions and potential involvement in cancer. RNA sequencing (RNA-seq) or quantitative reverse transcription-polymerase chain reaction (qRT-PCR) are the only validated methods to evaluate and study lncRNA expression and neither of them provides visual representation as immunohistochemistry (IHC) provides for proteins. We have standardized and are reporting a sensitive Z probe based in situ hybridization method to visually identify and quantify lncRNA in FFPE tissues. This assay is highly sensitive and identifies transcripts visible within different cell types and tumors. We have detected a scarcely expressed tumor suppressor lncRNA NRON (non-coding repressor of nuclear factor of activated T-cells (NFAT)), a moderately expressed oncogenic lncRNA UCA1 (urothelial cancer associated 1), and a highly studied and expressed lncRNA MALAT1 (metastasis associated lung adenocarcinoma transcript 1) in different cancers. High MALAT1 staining was found in colorectal, breast and pancreatic cancer. Additionally, we have observed an increase in MALAT1 expression in different stages of colorectal cancer.

Original languageEnglish (US)
Article number20
JournalNon-coding RNA
Volume4
Issue number3
DOIs
StatePublished - Sep 5 2018

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Long Noncoding RNA
Paraffin
Formaldehyde
Tissue
Neoplasms
Biomarkers
Neoplasm Metastasis
Tumors
Colorectal Neoplasms
RNA
NFATC Transcription Factors
RNA Sequence Analysis
Polymerase chain reaction
RNA Stability
Transcription
Pancreatic Neoplasms
Action Potentials
Reverse Transcription
In Situ Hybridization
Assays

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Z probe, an efficient tool for characterizing long non-coding RNA in FFPE tissues. / Tripathi, Manish; Zacheaus, Chidi; Doxtater, Kyle; Keramatnia, Fatemeh; Gao, Cuilan; Yallapu, Murali; Jaggi, Meena; Chauhan, Subhash.

In: Non-coding RNA, Vol. 4, No. 3, 20, 05.09.2018.

Research output: Contribution to journalArticle

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