Zinc supplementation suppresses the progression of bile duct ligation-induced liver fibrosis in mice

Fang Shi, Qin Sheng, Xinhua Xu, Wenli Huang, Yujian Kang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Metallothionein (MT) gene therapy leads to resolution of liver fibrosis in mouse model, in which the activation of collagenases is involved in the regression of liver fibrosis. MT plays a critical role in zinc sequestration in the liver suggesting its therapeutic effect would be mediated by zinc. The present study was undertaken to test the hypothesis that zinc supplementation suppresses liver fibrosis. Male Kunming mice subjected to bile duct ligation (BDL) resulted in liver fibrosis as assessed by increased α-smooth muscle actin (α-SMA) and collagen I production/deposition in the liver. Zinc supplementation was introduced 4 weeks after BDL surgery via intragastric administration once daily for 2 weeks resulting in a significant reduction in the collagen deposition in the liver and an increase in the survival rate. Furthermore, zinc suppressed gene expression of a-SMA and collagen I and enhanced the capacity of collagen degradation, as determined by the increased activity of total collagenases and elevated mRNA and protein levels of MMP13. Therefore, the results demonstrate that zinc supplementation suppresses BDL-induced liver fibrosis through both inhibiting collagen production and enhancing collagen degradation.

Original languageEnglish (US)
Pages (from-to)1197-1204
Number of pages8
JournalExperimental Biology and Medicine
Volume240
Issue number9
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Bile Ducts
Liver Cirrhosis
Liver
Ducts
Ligation
Zinc
Collagen
Metallothionein
Collagenases
Therapeutic Uses
Gene therapy
Degradation
Genetic Therapy
Smooth Muscle
Actins
Gene expression
Surgery
Muscle
Gene Expression
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zinc supplementation suppresses the progression of bile duct ligation-induced liver fibrosis in mice. / Shi, Fang; Sheng, Qin; Xu, Xinhua; Huang, Wenli; Kang, Yujian.

In: Experimental Biology and Medicine, Vol. 240, No. 9, 01.01.2015, p. 1197-1204.

Research output: Contribution to journalArticle

Shi, Fang ; Sheng, Qin ; Xu, Xinhua ; Huang, Wenli ; Kang, Yujian. / Zinc supplementation suppresses the progression of bile duct ligation-induced liver fibrosis in mice. In: Experimental Biology and Medicine. 2015 ; Vol. 240, No. 9. pp. 1197-1204.
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