Zonation of acetate labeling across the liver

Implications for studies of lipogenesis by MIDA

Michelle Puchowicz, Ilya R. Bederman, Blandine Comte, Dawei Yang, France David, Eric Stone, Kareem Jabbour, David H. Wasserman, Henri Brunengraber

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Measurement of fractional lipogenesis by mass isotopomer distribution analysis (MIDA) of fatty acids or cholesterol labeled from [13C]acetate assumes constant enrichment of lipogenic acetyl-CoA in all hepatocytes. This would not be the case if uptake and release of acetate by the liver resulted in transhepatic gradients of acetyl-CoA enrichment. Conscious dogs, prefitted with transhepatic catheters, were infused with glucose and [1,2- 13C2]acetate. Stable concentrations and enrichments of acetate were measured in artery (17 μM, 36%), portal vein (61 μM, 5.4%), and hepatic vein (17 μM, 1.0%) and were computed for mixed blood entering the liver (53 μM, 7.4%). We also measured balances of propionate and butyrate across gut and liver. All gut release of propionate and butyrate is taken up by the liver. The threefold decrease in acetate concentration and the sevenfold decrease in acetate enrichment across the liver strongly suggest that the enrichment of lipogenic acetyl-CoA decreases across the liver. Thus fractional hepatic lipogenesis measured in vivo by MIDA may be underestimated.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume277
Issue number6 40-6
StatePublished - Dec 1 1999

Fingerprint

Lipogenesis
Liver
Labeling
Acetates
Acetyl Coenzyme A
Butyrates
Propionates
Catheters
Hepatic Veins
Portal Vein
Blood
Fatty Acids
Cholesterol
Hepatocytes
Arteries
Glucose
Dogs

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Zonation of acetate labeling across the liver : Implications for studies of lipogenesis by MIDA. / Puchowicz, Michelle; Bederman, Ilya R.; Comte, Blandine; Yang, Dawei; David, France; Stone, Eric; Jabbour, Kareem; Wasserman, David H.; Brunengraber, Henri.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 277, No. 6 40-6, 01.12.1999.

Research output: Contribution to journalArticle

Puchowicz, M, Bederman, IR, Comte, B, Yang, D, David, F, Stone, E, Jabbour, K, Wasserman, DH & Brunengraber, H 1999, 'Zonation of acetate labeling across the liver: Implications for studies of lipogenesis by MIDA', American Journal of Physiology - Endocrinology and Metabolism, vol. 277, no. 6 40-6.
Puchowicz, Michelle ; Bederman, Ilya R. ; Comte, Blandine ; Yang, Dawei ; David, France ; Stone, Eric ; Jabbour, Kareem ; Wasserman, David H. ; Brunengraber, Henri. / Zonation of acetate labeling across the liver : Implications for studies of lipogenesis by MIDA. In: American Journal of Physiology - Endocrinology and Metabolism. 1999 ; Vol. 277, No. 6 40-6.
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